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| Catalog Code: | NB100-78369 |
| Product Name: | Rb Antibody |
| Product Description: | Rabbit Polyclonal anti-Rb |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant (partial), N-terminal |
| Cross Reactivity: | Mouse, Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000-1:10,000 dilutions. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Rb (retinoblastoma 1) is a 105 kD nuclear protein containing RB-associated protein A and B domains. Rb acts as a transcriptional negative regulator of G1-phase cell cycle progression, and sequesters E2F and other transcription factors. Rb is unphosphorylated and inactive in G0, and becomes active upon hypophosphorylation by cyclinD-cdk4/6 in early G1. Rb is inactive with hyperphosphorylated by cyclinE-cdk2 late G1. Hypophosphorylated Rb form complexes with E2F, HDAC, cyclinD-cdk4/6, cyclinE-cdk2, PU 1, ATF 2, UBF, Elf 1, and c-Abl. The Poly6146 antibody recognizes the N-terminal region of human and mouse Rb and has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence staining. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | immunofluorescence, immunoprecipitation, Western Blot |
| Species Summary: | Mu, Hu |
| Alternate Names: | anti-Retinoblastoma 1 antibody |
| Package Size: | 0.2 ml |
| General References: | 1. Knudson, A. G., Jr. 1986. Annu. Rev. Genet. 20:231. 2. Lee, E. et al., 1992. Nature 359:288. 3. Ezhevsky, S., et al., 2001. Mol. Cell Biol. 21:4773. 4. Narita, M., et al., 2003. Cell 113:703. |
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