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| Catalog Code: | NB100-78368 |
| Product Name: | RAD17 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Rad 17 [Ser645] |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Modified peptide |
| Cross Reactivity: | Mouse, Human, Reacts with Ser645-phosphorylated Rad17. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Rad17 (also known as Rad17 homolog and Rad17-like protein) has four reported isoforms FM1-FM4, with molecular weights of approximately 71 kD, 73 kD, 57 kD, and 62 kD, respectively. This nuclear protein functions as a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. Rad17 is a component of the checkpoint clamp loader complex (Rad17/Rfc2-5) and recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin. Rad17 can be modified by phosphorylation and has been shown to associate with chromatin, DNA replication factor C, ATR, and Hus1. The Poly6145 recognizes human, mouse, and rat phosphorylated Rad17 (Ser645) and has been shown to be useful for Western blotting. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | Western Blot |
| Species Summary: | Mu, Hu |
| Alternate Names: | anti-Rad17 homolog antibody, anti-Rad17-like protein antibody |
| Package Size: | 0.05 ml |
| Phosphorylated: | Ser645 |
| General References: | 1. Parker, A., et al., 1998. J. Biol. Chem. 273:18340. 2. Chen, M., et al., 2001. Gene. 277:145. 3. Bao, S., et al., 2001. Nature 411:969. 4. Wang, X., et al., 2003. Genes Dev. 17:965. |
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