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| Catalog Code: | NB100-78366 |
| Product Name: | PARP Antibody |
| Product Description: | Mouse Monoclonal anti-PARP (5A5), conjugated to Biotin |
| Clone: | 5A5 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Recombinant (partial), N-terminal 2/3 sequence of PARP |
| Cross Reactivity: | Mouse, Human. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular nick sensor and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Fin |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Mu, Hu |
| Alternate Names: | anti-Poly (ADP-ribose) polymerase antibody |
| Package Size: | 0.1 mg |
| Conjugate: | Biotin |
| General References: | 1. Cherney, B., et al., 1987. PNAS 84:8370. 2. Ikejima, M., et al., 1990. J. Biol. Chem. 265:21907. 3. Ying, W., et al., 2001. PNAS 98:12227. 4. Noel, G., et al., 2003. BMC Cell Biol. 4:7. |
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