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| Catalog Code: | NB100-78351 |
| Product Name: | DNA-PKcs Antibody |
| Product Description: | Mouse Monoclonal anti-DNA-PKcs (10B1) |
| Clone: | 10B1 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Modified peptide |
| Cross Reactivity: | Human, reacts with Thr2609-phosphorylated DNA-PKcs. |
| Packaging: | 0.1 mg protein G purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting. |
| Storage: | Upon receipt, store undiluted at 4 degC. |
| Purity: | protein G purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Fin |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-DNA-protein kinase catalytic subunit antibody, anti-DNA-activated protein kinase antibody, anti-DNA-dependent protein kinase antibody |
| Package Size: | 0.1 mg |
| General References: | 1. Hartley, K., et al., 1995. Cell. 82:849. 2. Connelly, M., et al., 1996. Gene. 175:271. 3. Douglas, P., et al., 2002. Biochem. J. 368:243 4. Calsou, P., et al., 2003. J. Mol. Biol. 326:93. |
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