| Catalog Code: | NB100-78350 |
| Product Type: | Primary Antibody |
| Product Name: | DNA-PKcs Antibody |
| List Price: | 285 |
| Clonality: | Monoclonal |
| Gene ID: | 5591 |
| Host: | Mouse |
| Clone: | 7A4 |
| Isotype: | IgG1, kappa |
| Product Description: | Mouse Monoclonal anti-DNA-PKcs (7A4) |
| Cross Reactivity: | Human. |
| Species Summary: | Hu(+) |
| Background: | DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. The 7A4 monoclonal antibody has recognizes human DNA-PKcs and has been shown to be useful for immunofluorescence staining and Western blotting. |
| Alternate Names: | anti-HB15 antibody |
| Immunogen: | Recombinant (partial) |
| Purity: | protein G purified |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Packaging: | 0.1 mg protein G purified Mouse antisera |
| PackageSize: | 0.1 mg |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Summary: | immunofluorescence, Western Blot |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| General References: | 1. Hartley, K., et al., 1995. Cell. 82:849. 2. Connelly, M., et al., 1996. Gene. 175:271. 3. Douglas, P., et al., 2002. Biochem. J. 368:243 4. Calsou, P., et al., 2003. J. Mol. Biol. 326:93. |
| WebLink: | www.novusbio.com/data_sheet/index/NB100 ... |
|