| request information: |
|
| Catalog Code: | NB100-78339 |
| Product Name: | APC10 Antibody |
| Product Description: | Rabbit Polyclonal anti-APC10 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant (partial), N-terminal |
| Cross Reactivity: | Mouse, Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | APC10 (anaphase-promoting complex subunit 10) is a member of the E3 enzyme family. This protein contains a Doc domain and has a molecular weight of approximately 21 kD. The APC10 protein is located at the centrosomes and mitotic spindles throughout mitosis and the kinetochores from prophase to anaphase, and at the midbody during telophase and cytokinesis. The APC10 protein is involved in substrate recognition and acts as a processivity factor. The APC10 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC10 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The Poly6115 antibody has been shown to be useful for Western blotting of the human and mouse APC10 protein. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | Western Blot |
| Species Summary: | Mu, Hu |
| Alternate Names: | anti-Anaphase-promoting complex subunit 10 antibody, anti-Doc1 antibody |
| Package Size: | 0.2 ml |
| General References: | 1. Kurasawa, Y., et al., 1999. Oncogene. 18:5131. 2. Zachariae, W., et al., 1999. Genes Dev. 13:2039. 3. Carroll, C., et al., 2002. Nat. Cell Biol. 4:880. 4. Passmore, L., et al., 2003. EMBO J. 22:786. |
order or
more information: | company product webpage |
|