| CatalogCode: | NB100-78334 |
| ProductName: | APC3 Antibody |
| Product Description: | Rabbit Polyclonal anti-APC3 |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant (partial), N-terminal |
| CrossReactivity: | Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Spindle poles, spindle microtubules, kinetochores, chromosome arms |
| Background: | APC3 (anaphase-promoting complex subunit 3) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 95 kD. The APC3 protein is located at the spindle poles, the spindle microtubules, kinetochores and chromosome arms. This protein is required for core stability and functions as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC3 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC3 protein interacts with Rb, Mad2, p55CDC, BUBR1, as well as the APC complex proteins noted above. The APC3 protein can be modified by phosphorylation by cdk1-cyclin B. The Poly6109 antibody has been shown to be useful for Western blotting of the human APC3 protein. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Anaphase-promoting complex subunit 3 antibody, anti-Cell division cycle protein 27 (Cdc27) antibody, anti-Nuc2 antibody |
| ProteinTarget: | APC3 |
| PackageSize: | 0.2 ml |
| GeneralRef: | 1. Chen, P., et al., 1995. Cell Growth Differ. 6:199. 2. King, R., et al., 1995. Cell 81:279. 3. Rudner, A., et al., 2000. J. Cell Biol. 149:1377. 4. Topper, L., et al., 2002. Cell Cycle. 1:282. |
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