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| Catalog Code: | NB100-78333 |
| Product Name: | APC2 Antibody |
| Product Description: | Rabbit Polyclonal anti-APC2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant (partial), N-terminal |
| Cross Reactivity: | Mouse, Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | APC2 (anaphase-promoting complex subunit 2) is a member of the E3 enzyme family. This protein contains cullin nbsprepeats and has a molecular weight of approximately 105 kD. The APC2 protein is located in the nucleus during interphase, and at the centrosome during metaphase/anaphase. This protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC2 protein functions as a probable catalytic subunit, a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC2 protein interacts with Rad51 (as well as the APC complex proteins noted above). The Poly6108 antibody has been shown to be useful for Western blotting of the human and mouse APC2 protein. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| Application Summary: | Western Blot |
| Species Summary: | Mu, Hu |
| Alternate Names: | anti-Anaphase-promoting complex subunit 2 antibody, anti-Cyclosome subunit 2 antibody |
| Package Size: | 0.2 ml |
| General References: | 1. Yu, H., et al., 1998. Science 279:1219. 2. Zachariae, W., et al., 1998. Science 279:1216. 3. Zachariae, W., et al., 1999. Genes Dev. 13:2039. 4. Tang, Z., et al., 2001. Mol. Biol. Cell 12:3839. |
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