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| Catalog Code: | NB100-78300 |
| Product Name: | Spt16 Antibody |
| Product Description: | Mouse Monoclonal anti-Spt16 (8D2) |
| Clone: | 8D2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Recombinant (partial) |
| Cross Reactivity: | Human. |
| Packaging: | 0.1 mg protein G purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Mouse |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2 |
| Application Summary: | immunofluorescence, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Facilitates Chromatin Transcription (FACT140) antibody, anti-Spt 16 protein antibody, anti-Cdc68 antibody, anti-anti-FACT antibody |
| Package Size: | 0.1 mg |
| Preservative: | Sodium Azide |
| General References: | 1. LeRoy, G., et al., 1998. Science 282:1836. 2. Orphanides, G., et al., 1998. Cell 92:105. 3. Orphanides, G., et al., 1999. Nature 400:284. |
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