| request information: |
|
| Catalog Code: | NB100-783 |
| Product Name: | ZCCHC11 Antibody |
| Product Description: | Goat Polyclonal anti-ZCCHC11 |
| Clonality: | Polyclonal |
| ListPrice: | 195 |
| Gene ID: | 23381 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide with sequence RERCPHPPRGNVSE, representing the C-Terminus of the protein (residues 1503-1516) according to BAA12105. |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody has can be used in Western Blot where a 250-300 kDa and 30-35 kDa band is seen in Raji Lysate. Recommended Starting Dilutions*:Western Blot: 1-3 ug/mlPlease note that the band we consistently detect in western blots differs from the predicted size of 181kDa. Currently we cannot find an explanation for this in the literature. However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].**Optimal dilutions should be determined by the end user. |
| Background: | **Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application. A ubiquitously expressed gene with a predicted C2H2 zinc-finger at its N-terminal region. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-KIAA0191 antibody, anti-zinc finger antibody, anti-CCHC domain containing 11antibody, anti-PAPD3 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Na azide |
| Notes Main: | **Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Uses & Dilutions below). LocusLink ID - 23318 |
| General References: | b>Nagase T et al. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res 3:17-24, 1996. |
order or
more information: | company product webpage |