| request information: |
|
| Catalog Code: | NB100-78265 |
| Product Name: | p27Kip1 Antibody |
| Product Description: | Rabbit Polyclonal anti-p27Kip1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Peptide derived from the carboxy terminus of human p27 |
| Cross Reactivity: | Human. |
| Packaging: | 0.2 ml protein A purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | p27Kip1 (cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor p27) is a 27kD cyclin-dependent inhibitor. p27Kip1 is localized in the nucleus and cytoplasm (phospho-specific). This protein functions in G1 arrest and binds and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2. p27Kip1 mediates TGF beta-induced G1 arrest and regulates cell motility. This kinase has also been implicated in translational control. Akt phosphorylation causes p27 Kip1 export to cytoplasm; p27 Kip1 has also been shown to be ubiquitinated. p27 Kip1 has been shown to interact with NUP50, Skp2, and PKB/Akt. The Poly6029 antibody recognizes the N-terminal region of human p27Kip1 and has been shown to be useful for Western blotting. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Cyclin-dependent kinase inhibitor 1B antibody, anti-Cyclin-dependent kinase inhibitor p27 antibody, anti-p27 kip1 antibody |
| Package Size: | 0.2 ml |
| General References: | 1. Polyak, K., et al., 1994. Cell 78:59. 2. Millard, S., et al., 2000. Mol. Cell. Biol. 20:5947. 3. Viglietto, G., et al., 2002. Nat. Med. 8:1136. 4. McAllister, S., et al., 2003. Mol. Cell. Biol. 23:216. |
order or
more information: | company product webpage |