| CatalogCode: | NB100-78251 |
| ProductName: | Cdc25C Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdc25C |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant (partial), N-terminal |
| CrossReactivity: | Human. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Nuclear |
| Background: | Cdc25C (also known as cell division cycle 25C protein, mitosis inducer Cdc25, and dual specificity phosphatase Cdc25C) is a MPI phosphatase containing a rhodanese domain. There are multiple isoforms of this phosphatase C1-5 with apparent molecular weights of 53-56 kD (alternatively spliced transcript variants). Cdc25C functions as a nuclear dosage-dependent inducer of mitotic control required for progression of cell cycle. This protein dephosphorylates cyclin B-bound Cdc2 and suppresses p53-induced growth arrest. Cdc25C activity is inhibited by Chk1 phosphorylation. Cdc25C has been shown to interact with Cyclin B, Cdc2, Chk1, and 14-3-3. The Poly6013 antibody has been shown to be useful for Western blotting of the human Cdc25C protein. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Cell division cycle 25C protein antibody, anti-Mitosis inducer Cdc25 antibody, anti-Dual specificity phosphatase Cdc25C antibody |
| ProteinTarget: | Cdc25C |
| PackageSize: | 0.2 ml |
| GeneralRef: | 1. Sartor, H., et al., 1992. Genomics 13:911. 2. Zwicker, J., et al., 1995. Prog. Cell Cycle 1:91. 3. Turowski, P., 2003. Mol. Biol. Cell. 14:2984. 4. Sohn, J., 2003. Biochemistry. 42:10060. |