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| Catalog Code: | NB100-78238 |
| Product Name: | IL-8 Antibody |
| Product Description: | Mouse Monoclonal anti-IL-8 (H8A5) |
| Clone: | H8A5 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Cross Reactivity: | Human. |
| Packaging: | 0.5 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The H8A5 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA capture antibody. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA, Immunocytochemistry, flow cytometry / FACS analysis |
| Species Summary: | Hu |
| Alternate Names: | anti-Interleukin-8 antibody, anti-Neutrophil activating protein-1 antibody, anti-Monocyte-derived neutrophil chemotactic factor (MDNCF) antibody, anti-Neutrophil activating factor (NAF) antibody, anti-Leukocyte adhesion inhibitor (LAI) antibody, anti-Granulocyte chemotactic protein (GCP) antibody, anti-CXCL8 antibody |
| Package Size: | 0.5 mg |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: H8A5 - E8N1 For Sandwich ELISA pair this antibody with: NB100-78236 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Baggiolini, M., et al., 1994. Adv. Immunol. 55:97. 3. Schr oumlder, J., et al., 1992. Immunology Ser. 57:387. |
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