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| Catalog Code: | NB100-78225 |
| Product Name: | IL-12 Antibody |
| Product Description: | Mouse Monoclonal anti-IL-12 (C8.6), conjugated to Biotin |
| Clone: | C8.6 |
| Clonality: | Monoclonal |
| ListPrice: | 315 |
| Immunogen: | COS-expressed p40 subunit of human IL-12 |
| Cross Reactivity: | Human, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40). |
| Packaging: | 0.5 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 mug/ml is recommended. nbsp To obtain a linear standard curve, serial dilutions of IL-12 p40 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. nbsp To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. nbsp For use as an ELISPOT detection antibody, a concentration range of 1-4 mug/ml is recommended. nbsp It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | The C8.6 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C8.6 antibody has been reported to strongly inhibit different biological activities of IL-12, (e.g., IFN-gamma induction, mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity). The C8.6 antibody can neutralize the bioactivity of natural or recombinant IL-12. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA |
| Species Summary: | Hu |
| Alternate Names: | anti-Interleukin-12 p40 antibody, anti-Interleukin-23 p40 antibody, anti-Cytotoxic lymphocyte maturation factor (CLMF) antibody, anti-Natural killer cell stimulatory factor (NKSF) antibody, anti-CTL maturation factor (TcMF) antibody, anti-T-cell stimulating factor (TSF) antibody |
| Package Size: | 0.5 mg |
| Conjugate: | Biotin |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: C8.3 - C8.6 For Sandwich ELISA pair this antibody with: NB100-78147 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux, V. 1992. Research Immunol. 143:385. 3. Trinchieri, G., et al., 1992 Prog. Growth Factor Res. 4:355. |
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