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| Catalog Code: | NB100-78211 |
| Product Name: | IL-17, IL-17A Antibody |
| Product Description: | Rat Monoclonal anti-IL-17, IL-17A (TC11-18H10.1) |
| Clone: | TC11-18H10.1 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | E. coli-expressed, recombinant mouse IL-17A |
| Cross Reactivity: | Mouse. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 2-6 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; activation of NF- kappaB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-18H10.1 antibody can neutralize IL-17A activity. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA, ELISPOT Capture, Immunocytochemistry, flow cytometry / FACS analysis, Western Blot |
| Species Summary: | Mu |
| Alternate Names: | anti-Interleukin-17 antibody, anti-Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) antibody, anti-CTLA 8 antibody, anti-IL17 antibody, anti-IL 17 antibody |
| Package Size: | 0.5 mg |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: TC11-18H10.1 - TC11-8H4 For Sandwich ELISA pair this antibody with: NB100-78213 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Numasaki, M., et al., 2002. Blood 101:2620. 3. Fossiez, F., et al., 1996. J. Exp. Med. 183:2593. |
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