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| Catalog Code: | NB100-78200 |
| Product Name: | TNF- alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-TNF- alpha, conjugated to Biotin |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Mouse, Rat. |
| Packaging: | 4 Plates Immunogen affinity purified Rabbit antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection, the suggested use of this reagent is 1:1000 dilution. For use as an ELISPOT detection antibody, the range of 1:250-1:500 dilution is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF- alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate or serial dilutions of rat TNF- alpha recombinant protein ranging from 2500 to 20 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomeric mouse TNF- alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF- alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF- alpha has also been described. TNF- alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The Poly5062 antibody reacts with mouse and rat tumor necrosis factor- alpha (TNF- alpha). |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.5% BSA (USA origin) and 0.05% sodium azide. |
| Application Summary: | ELISA |
| Species Summary: | Mu, Rt |
| Alternate Names: | anti-Tumor necrosis factor-alpha antibody, anti-Cachectin antibody, anti-Necrosin antibody, anti-Macrophage cytotoxic factor (MCF) antibody, anti-Differentiation inducing factor (DIF) antibody, anti-TNFSF-2 antibody, anti-TNF-a antibody, anti-TNF-alpha antibody |
| Package Size: | 4 Plates |
| Conjugate: | Biotin |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Beutler, B., et al., 1988. Annu. Rev. Biochem. 57:505. 3. Beutler, B., et al., 1989. Annu. Rev. Immunol. 7:625. |
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