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| Catalog Code: | NB100-78182 |
| Product Name: | IL-10 Antibody |
| Product Description: | Rat Monoclonal anti-IL-10 (JES5-16E3) |
| Clone: | JES5-16E3 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | E. coli-expressed, recombinant mouse IL-10 |
| Cross Reactivity: | Mouse. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.1-1.0 mug/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Immunohistochemistry-frozen/paraffin. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For ELISA capture, Carbonate buffer, pH 9.5 is highly recommended for coating buffer. |
| Background: | IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). The JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2b, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA, Immunocytochemistry, flow cytometry / FACS analysis, immunohistochemistry |
| Species Summary: | Mu |
| Alternate Names: | anti-Interleukin-10 antibody, anti-Cytokine synthesis inhibitory factor (CSIF) antibody, anti-B cell derived T cell growth factor (B-TCGF) antibody, anti-T cell growth inhibitory factor (TGIF) antibody |
| Package Size: | 0.5 mg |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: JES5-16E3 - JES5-2A5 For Sandwich ELISA pair this antibody with: NB100-78181 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. de Waal-Malefy, T, et al., 1992. Curr. Opin. Immunol. 4:31 |
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