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| Catalog Code: | NB100-78171 |
| Product Name: | IL-3 Antibody |
| Product Description: | Rat Monoclonal anti-IL-3 (MP2-8F8) |
| Clone: | MP2-8F8 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | COS-expressed, recombinant mouse IL-3 |
| Cross Reactivity: | Mouse. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-3 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA, Immunocytochemistry, flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation |
| Species Summary: | Mu |
| Alternate Names: | anti-Interleukin-3 antibody, anti-Burst promoting activity (BP/BPA) antibody, anti-Eosinophil colony stimulating factor (Eo-CSF) antibody, anti-Hematopoietic cell growth factor (HCGF) antibody, anti-Mast cell growth factor (MGF/MCGF) antibody, anti-Multi-colony stimulating factor (Multi-CSF) antibody, anti-P cell stimulating activity (PCSA), antibody |
| Package Size: | 0.5 mg |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: MP2-8F8 - MP2-43D11 For Sandwich ELISA pair this antibody with: NB100-78172 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421. 3. Ihle, J., 1992. Chem. Immunology 51:65. |
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