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| Catalog Code: | NB100-78160 |
| Product Name: | MCP-1 Antibody |
| Product Description: | Mouse Monoclonal anti-MCP-1 (5D3-F7), conjugated to Biotin |
| Clone: | 5D3-F7 |
| Clonality: | Monoclonal |
| ListPrice: | 340 |
| Immunogen: | Recombinant human MCP-1 |
| Cross Reactivity: | Human, Expected Cross-Reactivity based on immunogen sequence: Cynomolgus, Rhesus. |
| Packaging: | 0.5 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1). |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA, Immunocytochemistry, flow cytometry / FACS analysis |
| Species Summary: | Hu, Mk |
| Alternate Names: | anti-Monocyte chemoattractant protein-1 antibody, anti-Monocyte chemoattractant and activating factor (MCAF) antibody, anti-JE antibody, anti-Small inducible cytokine A2 (SCYA2) antibody, anti-HC-11 antibody, anti-P6 antibody, anti-Smooth muscle cell chemotactic factor (SMC-CF) antibody, anti-CCL2 antibody |
| Package Size: | 0.5 mg |
| Conjugate: | Biotin |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: 2H5 - 5D3-F7 For Sandwich ELISA pair this antibody with: NB100-78196 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff, S., et al., 1992. J. Exp. Med. 175:1271. 3. Charo, I., et al., 199 |
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