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| Catalog Code: | NB100-78144 |
| Product Name: | IL-10 Antibody |
| Product Description: | Rat Monoclonal anti-IL-10 (JES3-12G8), conjugated to Biotin |
| Clone: | JES3-12G8 |
| Clonality: | Monoclonal |
| ListPrice: | 315 |
| Immunogen: | COS - expressed, recombinant human IL-10 |
| Cross Reactivity: | Human. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 mug/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-12G8 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-12G8 antibody can neutralize the bioactivity of natural or recombinant IL-10. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA |
| Species Summary: | Hu |
| Alternate Names: | anti-Interleukin-10 antibody, anti-B cell derived T cell growth factor (B-TCGF) antibody, anti-Cytokine synthesis inhibitory factor (CSIF) antibody, anti-T-cell growth inhibitory factor (TGIF) antibody |
| Package Size: | 0.5 mg |
| Conjugate: | Biotin |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: JES3-9D7 - JES3-12G8 For Sandwich ELISA pair this antibody with: NB100-78143 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. de Waal-Malefyt, R., et al., 1992. Curr. Opin. Immunol. 4:31 |
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