| CatalogCode: | | NB100-78143 |
| ProductName: | | IL-10 Antibody |
| Product Description: | | Rat Monoclonal anti-IL-10 (JES3-9D7) |
| Clone: | | JES3-9D7 |
| Clonality: | | Monoclonal |
| Immunogen: | | COS - expressed, recombinant human IL-10 |
| CrossReactivity: | | Human, Expected Cross-Reactivity based on immunogen sequence: Baboon, Rhesus. |
| Packaging: | | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | | Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | | T cells, B cells, mast cells, macrophages |
| Background: | | IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10. |
| Storage: | | Store at 4C. Do not freeze. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG1, kappa |
| Host_Name: | | Rat |
| Buffer: | | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| ListPrice: | | 285 |
| AppSummary: | | ELISA, ICC, FACS, IHC, WB |
| SpeciesSummary: | | Hu, Bb |
| ALTnames: | | anti-Interleukin-10 antibody, anti-B cell derived T cell growth factor (B-TCGF) antibody, anti-Cytokine synthesis inhibitory factor (CSIF) antibody, anti-T-cell growth inhibitory factor (TGIF) antibody |
| ProteinTarget: | | IL-10 |
| PackageSize: | | 0.5 mg |
| GeneralRef: | | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. de Waal-Malefyt, R., et al., 1992. Curr. Opin. Immunol. 4:31 |
| more information: | | company product webpage |
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