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| Catalog Code: | NB100-78127 |
| Product Name: | IL-3 Antibody |
| Product Description: | Rat Monoclonal anti-IL-3 (BVD3-1F9) |
| Clone: | BVD3-1F9 |
| Clonality: | Monoclonal |
| ListPrice: | 315 |
| Immunogen: | Yeast-expressed, recombinant human IL-3 |
| Cross Reactivity: | Human. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by ELISA assay. The purified BVD3-1F9 has been tested by blocking fluorochrome conjugated BVD3-1F9 for intracellular cytokine staining. Immunohistochemistry-frozen/paraffin. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | Immunocytochemistry, flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Interleukin-3 antibody, anti-Burst promoting activity (BP/BPA) antibody, anti-Eosinophil colony stimulating factor (Eo-CSF) antibody, anti-Hematopoietic cell growth factor (HCGF) antibody, anti-Mast cell growth factor (MGF/MCGF) antibody, anti-Multi-colony stimulating factor (Multi-CSF) antibody, anti-P cell stimulating activity (PCSA) antibody |
| Package Size: | 0.5 mg |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421. 3. Ihle, J., 1992. Chem. Immunology 51:65. |
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