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| Catalog Code: | NB100-781 |
| Product Name: | SOCS1 Antibody |
| Product Description: | Goat Polyclonal anti-SOCS1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Gene ID: | 8651 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide with sequence VLRDYLSSFPFQI, representing the C-Terminus of the protein (residues 199-211) according to NP_003736 |
| Cross Reactivity: | Cross-reacts with Mouse. Expected to cross-react with Human due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for Western Blot where a 30-35 kDa band is seen in Mouse Spleen Lysate. Recommended Dilutions*:Western Blot: 0.5-2 ug/ml.Please note that although the predicted size of SOCS1 is 24kDa according to NP_003736, Chen et al., [PNAS, Feb 2002; 99: 2175 - 2180] and Lindeman et al., [Genes & Dev., Jul 2001; 15: 1631 - 1636] observe SOCS1 migrating at approx 30kDa on SDS-PAGE.**Optimal dilutions should be determined by the end user. |
| Background: | Cytokines induce a variety of biologic responses by binding to specific cell surface receptors and activating cytoplasmic signal transduction pathways. Proteins that mediate these signals from the cell surface to the nucleus include Janus kinases, e.g., Janus kinase-1, and the signal transducers and activators of transcription (STATs), such as STAT1. To maintain tight control of these signal transduction pathways, mechanisms for turning off the signals are necessary. The adverse consequences of an imbalance between positive and negative signals is evident in the 'motheaten' mouse, which lacks the phosphatase SHP1. These mice fail to regulate cytokine responses appropriately, resulting in hyperproliferation and accumulation of cells in several hematopoietic lineages and the development of systemic autoimmune disease. SOCS1 represents a family of proteins that regulates responses to cytokines. SOCS1 (also known as Janus kinase-binding protein and STAT-induced STAT inhibitor-1) was isolated independently by its ability to interact with Janus kinase-2 in a 2-hybrid screen and on the basis of antigenic similarity with the SH2 domain of STAT3. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine-inducible SH2-containing protein). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| Application Summary: | Western Blot |
| Species Summary: | Mu |
| Alternate Names: | anti-CISH1 antibody, anti-Cytokine Inducible SH2 Protein 1 antibody, anti-JAB antibody, anti-JAK Binding Protein antibody, anti-SOCS 1 antibody, anti-SSI1 antibody, anti-STAT Induced STAT Inhibitor 1 antibody, anti-Supressor of cytokine Signalling 1 antibody, anti-TEC Interacting Protein 3 antibody, anti-TIP3 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Na azide |
| Notes Main: | LocusLink ID - 8651 |
| General References: | 1. Starr, R., et al. A family of cytokine-inducible inhibitors of signalling. Nature. 387:917-21, 1997. |
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