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| Catalog Code: | NB100-78010 |
| Product Name: | CD99 Antibody |
| Product Description: | Mouse Monoclonal anti-CD99 (HCD99), conjugated to Biotin |
| Clone: | HCD99 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Human. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | The HCD99 monoclonal antibody recognizes human CD99 also known as CD99 antigen, E2 antigen, MIC2, and T-cell surface glycoprotein E2. CD99 is a type I, single chain transmembrane protein devoid of N-linked glycosylation sites that is encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, T cell leukemias and lymphomas and is absent on fetal B cells, some B cell lines, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H. The HCD99 antibody has been reported to be useful for flow cytometric detection of human CD99 and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues). |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu |
| Alternate Names: | anti-E2 antigen antibody, anti-MIC2 antibody, anti-T-cell surface glycoprotein E2 antibody |
| Package Size: | 0.1 mg |
| Conjugate: | Biotin |
| General References: | 1. Gelin, C., et al. 1989. EMBO J. 8:3253.2. Goodfellow, P.J., et al. 1986. Science 234:740.3. Pettersen, R.D., et al. 2001. J. Immunol. 166:4931. |
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