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| Catalog Code: | NB100-77967 |
| Product Name: | CD53 Antibody |
| Product Description: | Mouse Monoclonal anti-CD53 (MEM-53), conjugated to FITC |
| Clone: | MEM-53 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Leucocytes from a patient with LGL-type leukemia |
| Cross Reactivity: | Human. |
| Packaging: | 100 tests Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microliter per million cells or 20 microliter per 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | CD53 is a 35-42 kD type III tetraspan membrane protein. It is expressed on all leukocytes, but not on platelets or erythrocytes. CD53 is thought to be involved in signal transduction. It has been shown to interact with a number of proteins including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highly glycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein by approximately 25 kD. The MEM-53 antibody has been shown to be useful for flow cytometry, Western blotting, immunoprecipitation, immunohistochemistry (frozen), and functional activation of cells. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu |
| Package Size: | 100 tests |
| Conjugate: | FITC |
| General References: | 1. Amiot, M. 1990. J. Immunol. 145:4322.2. Angelisova, P., et al., 1990. Immunogenetics 32:281.3. Olweus, J., et al., 1993. J. Immunol. 153:4997. |
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