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| Catalog Code: | NB100-77869 |
| Product Name: | TWEAK Antibody |
| Product Description: | Mouse Monoclonal anti-TWEAK (CARL-2) |
| Clone: | CARL-2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | hTWEAK/2PK-3 transfectant cells |
| Cross Reactivity: | Human. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by sandwich ELISA. For ELISA capture applications, a concentration range of 1-4 mug/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF- kappaB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. The CARL-2 antibody can neutralize the bioactivity of natural or recombinant TWEAK. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgM, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | ELISA, flow cytometry / FACS analysis |
| Species Summary: | Hu |
| Alternate Names: | anti-TNF-like weak inducer of apoptosis antibody, anti-Apo-3 ligand (Apo3L) antibody, anti-Death receptor 3-L (DR3-L) antibody, anti-TNFSF 12 antibody, anti-CD255 antibody |
| Package Size: | 0.1 mg |
| Notes Main: | This antibody can be used in a sandwich ELISA (coating - conjugate) with the following clone pair: CARL-2 - CARL-1 For Sandwich ELISA pair this antibody with: NB100-77868 |
| General References: | 1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Chicheportiche, Y., et al., 1997. J. Biol. Chem. 272:32401. 3. Kaplan, M., et al., 2000. J. Immunol. 164:2897. |
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