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| Catalog Code: | NB100-77815 |
| Product Name: | CD86 Antibody |
| Product Description: | Mouse Monoclonal anti-CD86 (IT2.2) |
| Clone: | IT2.2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Human, Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon, Cynomolgus, Rhesus. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, known as CTLA-4, to deliver an inhibitory signal to T cells. The IT2.2 antibody blocks T cell activation. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2b, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis , Western Blot , Immunohistochemistry-Frozen |
| Species Summary: | Hu, Bb, Mk |
| Alternate Names: | anti-B7-2 antibody, anti-B7 2 antibody, anti-B70 antibody |
| Package Size: | 0.1 mg |
| General References: | 1. Hathcock, K., et al., 1996. Adv. Immunol. 62:131. 2. June, C., et al., 1994 |
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