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| Catalog Code: | NB100-77778 |
| Product Name: | CD28 Antibody |
| Product Description: | Mouse Monoclonal anti-CD28 (CD28.2), conjugated to FITC |
| Clone: | CD28.2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Human, Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon, Cynomolgus, Rhesus. |
| Packaging: | 100 tests Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microliter per million cells or 20 microliter per 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subset, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered as a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu, Bb, Mk |
| Alternate Names: | anti-T44 antibody, anti-Tp44 antibody |
| Package Size: | 100 tests |
| Conjugate: | FITC |
| General References: | 1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June, C.H., et al., 199 |
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