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| Catalog Code: | NB100-77777 |
| Product Name: | CD28 Antibody |
| Product Description: | Mouse Monoclonal anti-CD28 (CD28.2), conjugated to Biotin |
| Clone: | CD28.2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Human, Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon, Cynomolgus, Rhesus. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subset, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered as a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG1, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Hu, Bb, Mk |
| Alternate Names: | anti-T44 antibody, anti-Tp44 antibody |
| Package Size: | 0.1 mg |
| Conjugate: | Biotin |
| General References: | 1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June, C.H., et al., 199 |
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