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| Catalog Code: | NB100-77712 |
| Product Name: | CD8a Antibody |
| Product Description: | Mouse Monoclonal anti-CD8a (G28) |
| Clone: | G28 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | CD8+ Wistar rat splenic T cell hybridomas |
| Cross Reactivity: | Rat. |
| Packaging: | 0.5 mg Immunogen affinity purified Mouse antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8 alpha. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8 beta chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 alpha beta heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis , immunoprecipitation , Immunohistochemistry-Frozen |
| Species Summary: | Rt |
| Alternate Names: | anti-CD8a antibody, anti-T8 antibody, anti-Leu-8 antibody, anti-Ly-2antibody, anti-Ly2 antibody, anti-Lyt2 antibody, anti-Lyt-2 antibody |
| Package Size: | 0.5 mg |
| General References: | 1. Brideau, R.J., et al., 1980. Eur. J. Immunol. 10:609. 2. Dallman, M.J., et al., 1982. Eur. J. Immunol. 12:511. 3. Cantrell, D.A., et al., 1982. Immunol. 45:97. |
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