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| Catalog Code: | NB100-77700 |
| Product Name: | F4/80 Antibody |
| Product Description: | Rat Monoclonal anti-F4/80 (BM8), conjugated to FITC |
| Clone: | BM8 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Cross Reactivity: | Mouse. |
| Packaging: | 0.05 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8 T cells-mediated peripheral tolerance. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Mu |
| Alternate Names: | anti-EMR1 antibody, anti-Ly71 antibody, anti-Ly-71 antibody |
| Package Size: | 0.05 mg |
| Conjugate: | FITC |
| General References: | 1. Austy, J.M. and Gordon, S. 1981. Eur. J.Immunol. 11:805.2. Hume, DA., et al., 1983. J. Exp. Med. 158(5):1522.3. Ruedl, C., et al. 1996. Eur. J. Immunol. 26(8):1801. |
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