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| Catalog Code: | NB100-77669 |
| Product Name: | Panendothelial Cell Ag Antibody |
| Product Description: | Rat Monoclonal anti-Panendothelial Cell Ag (MECA-32), conjugated to Biotin |
| Clone: | MECA-32 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Mouse lymph node stromal cells |
| Cross Reactivity: | Mouse. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume. Immunohistochemistry. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after. |
| Storage: | Store at 4C. Do not freeze. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis, Immunocytochemistry, immunohistochemistry |
| Species Summary: | Mu |
| Alternate Names: | anti-Panendothelial Cell Antigen antibody, anti-MECA-32 antibody, anti-MECA32 antibody |
| Package Size: | 0.5 mg |
| Conjugate: | Biotin |
| General References: | 1. Leppnik, D.M., et al., 1989. Transplantation 48: 874.2. Hallmann, R., et al. 1995. Dev. Dyn. 202: 325.3. Kruse, A., et al., 1999. Bio. Reprod. 61: 1393. |
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