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| Catalog Code: | NB100-77510 |
| Product Name: | I-A/I-E Antibody |
| Product Description: | Rat Monoclonal anti-I-A/I-E (M5/114.15.2), conjugated to FITC |
| Clone: | M5/114.15.2 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Activated C57BL/6 mouse spleen cells |
| Cross Reactivity: | Mouse, Reacts with a shared determinant on I-Ab,d,q, and I-Ed,k alloantigens.. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2p,r haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2b, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis, Immunocytochemistry, flow cytometry / FACS analysis |
| Species Summary: | Mu |
| Alternate Names: | anti-MHC class II antibody |
| Package Size: | 0.5 mg |
| Conjugate: | FITC |
| General References: | 1. Watts, C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer, E., et al., 1998. Ann. Rev. Immunol. 16:323. |
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