| CatalogCode: | NB100-77482 |
| ProductName: | CD123 Antibody |
| Product Description: | Rat Monoclonal anti-CD123 (5B11) |
| Clone: | 5B11 |
| Clonality: | Monoclonal |
| Immunogen: | CTLL cells transfected with the mouse IL-3R alpha chain |
| CrossReactivity: | Mouse. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Control: | Hematopoietic progenitors, basophils, mast cells, megakaryocytes |
| Background: | CD123 is a 70 kD alpha chain subunit of the IL-3 receptor (IL-3R alpha). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common beta chain (CDw131) or the IL-3R beta chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the alpha subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R beta subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host_Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| ListPrice: | 285 |
| AppSummary: | FACS |
| SpeciesSummary: | Mu |
| ALTnames: | anti-IL3 Receptor alpha antibody, anti-IL-3 Receptor alpha antibody, anti-IL3-R alpha antibody, anti-IL-3R alpha antibody |
| ProteinTarget: | CD123 |
| PackageSize: | 0.5 mg |
| GeneralRef: | 1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Miyajima, A., et al., 1993. Blood 82:1960. 3. Itoh, N., et al., 1990. Science 247:324. |
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