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| Catalog Code: | NB100-77445 |
| Product Name: | CD62L Antibody |
| Product Description: | Rat Monoclonal anti-CD62L (MEL-14) |
| Clone: | MEL-14 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | C3H/eb mouse B lymphoma 38C-13 |
| Cross Reactivity: | Mouse. |
| Packaging: | 0.5 mg Immunogen affinity purified Rat antisera |
| Uses: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry. It is recommended that the reagent be titrated for optimal performance for each application. |
| Background: | CD62L is a 74-95 kD glycoprotein, also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte rolling on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naive, effector, and memory T cells. CD62L has been reported to interact with CD34, glyCAM-1, and MAdCAM-1. The MEL-14 antibody is able to block the binding of lymphocytes to peripheral lymph node HEV in vitro and to inhibit lymphocyte extravasation in vivo. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a, kappa |
| Host Name: | Rat |
| Buffer: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation |
| Species Summary: | Mu |
| Alternate Names: | anti-LECAM-1 antibody, anti-Ly22 antibody, anti-Ly-22 antibody, anti-LAM-1 antibody, anti-MEL-14 antibody, anti-L Selectin antibody, anti-LAM1 antibody |
| Package Size: | 0.5 mg |
| General References: | 1. Barclay, A.N., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Kishimoto, T.K, et al., 1990. PNAS 87:224 |
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