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| Catalog Code: | NB100-77336 |
| Product Name: | CRYAB Antibody |
| Product Description: | Goat Polyclonal anti-CRYAB |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Gene ID: | 1410 (human); 12955 (mouse); 25420 (rat); |
| Gene Symbol: | CRYAB |
| Reference Sequence: | NP_001876.1 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide, C-RLEKDRFSVNLD, representing the internal region of the sequence according to NP_001876.1 |
| Epitope: | RLEKDRFSVNLD |
| Cross Reactivity: | Human, mouse. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 18-19kDa band observed in Human Muscle and Mouse Eye lysates (calculated MW of 20.2kDa according to Human NP_001876.1 and 20.1kDa according to Mouse NP_034094.1). Recommended concentration: 0.01-0.03ug/ml. |
| Localization: | Cytoplasmic |
| Background: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Tris-saline [pH7.3] with 0.5% BSA |
| Application Summary: | ELISA 1:128000, Western Blot 0.01-0.03ug/ml |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-CRYAB antibody, anti-crystallin, alpha B antibody, anti-CRYA2 antibody, anti-CTPP2 antibody, anti-HSPB5 antibody, anti-heat-shock 20 kD like-protein antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% sodium azide |
| Homology: | Human, Mouse, Rat |
| General References: | Ousman SS, Tomooka BH, van Noort JM, Wawrousek EF, O'conner K, Hafler DA, Sobel RA, Robinson WH, Steinman L.Protective and therapeutic role for alphaB-crystallin in autoimmune demyelination.Nature. 2007 Jun 13; [Epub ahead of print]PMID: 17568699 |
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