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| Catalog Code: | NB100-769 |
| Product Name: | BCL 7A Antibody |
| Product Description: | Goat Polyclonal anti-BCL 7A |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Gene ID: | 605 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic peptide representing the C-terminus of the human protein (residues 219-231) sequence MKLEASQQNSEEM, according to NP_066273 |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Mouse due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | This antibody can be used for Western Blot, where a 35 kDa band can be seen in HeLa cell lysates. Recommended Starting Dilution:WB: 0.5-2ug/ml.**Please note that BCL7A, according to NP_066273, has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. This may be the reason why we see an approx 35kDa band rather than the predicted 26kDa band. |
| Background: | This gene is directly involved--with Myc and IgH--in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | Tris saline containing [pH7.3] with 0.5% BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-B-cell CLL/lymphoma 7 antibody, anti-B-cell CLL/lymphoma 7A antibody, anti-BCL7 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Na azide |
| Notes Main: | This protein is similar to the actin-binding protein caldesmon. |
| General References: | 1. Zani, V.J., et al. Molecular cloning of complex chromosomal translocation t(8;14;12)(q24.1;q32.3;q24.1) in a Burkitt lymphoma cell line defines a new gene (BCL7A) with homology to caldesmon. Blood. 87:3124-34, 1996. |
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