| CatalogCode: | NB100-697 |
| ProductName: | ETS1 Antibody |
| Product Description: | Mouse Monoclonal anti-ETS1 (1G11) |
| Clone: | 1G11 |
| Clonality: | Monoclonal |
| Immunogen: | Prokaryotic recombinant protein corresponding to amino acids 226 to 454 of the human Ets-1 oncoprotein. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.25 ml Tissue culture supernatant Mouse ascites. |
| Uses: | IHC: Use at a dilution of 1/15 - 1/25. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining.WB: Use at a dilution of 1/100-1/500.IP: Use at an assay dependent dilution.Not tested in other applications.*Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Control: | Tonsil tissue sections. |
| Background: | The ETS1 is a transcription factor known to regulate expression of a number of genes involved in extracellular matrix remodeling. ETS1 is absent from normal gastric epithelium but is expressed in approximately 60% of gastric carcinomas and oral squamous cell carcinomas with a significant correlation to the tumor stage. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Tissue Culture Supernatant |
| ListPrice: | 285 |
| AppSummary: | IHC, WB, IP |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1 antibody, anti-C-ets-1 protein antibody, anti-p54 antibody, anti-v-ets erythroblastosis virus E26 oncogene homolog 1 antibody |
| ProteinTarget: | ETS1 antibody [1G11] |
| PackageSize: | 0.25 ml |
| GeneralRef: | 1. Pande P et al. Ets-1: a plausible marker of invasive potential and lymph node metastasis in human oral squamous cell carcinomas. J Pathol 189:40-5 (1999). 2. Nakayama T et al. Expression of the Ets-1 proto-oncogene in human gastric carcinoma: correlation with tumor invasion. Am J Pathol 149:1931-9 (1996). 3. Bories JC et al. Increased T-cell apoptosis and terminal B-cell differentiation induced by inactivation of the Ets-1 proto-oncogene. Nature 377:635-8 (1995). 4. Wernert N et al. Stromal expression of c-Ets1 transcription factor correlates with tumor invasion. Cancer Res 54:5683-8 (1994). |