| CatalogCode: | NB100-695 |
| ProductName: | VDAC1 Antibody |
| Product Description: | Rabbit Polyclonal anti-VDAC1 - Mitochondrial Marker |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide corresponding to amino acid residues 185-197 of human VDAC1/Porin. |
| Specificity: | This antibody reacts with all forms of VDAC, including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). |
| CrossReactivity: | Human and rat. Expected to react with mouse and other species, including avian, mammmalian, aquatic and reptilian based on sequence homology. Other species have not been tested. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for Western blot and ELISA. By Western Blot, a band is detected between 30-33 kDa. This antibody can be blocked with VDAC1 peptide. |
| Localization: | Mitochondria, cell membrane |
| Background: | VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 0.02 M Potassium phoshate, 0.15 M sodium chloride, [pH 7.2] |
| ListPrice: | 285 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-Voltage-dependent anion-selective channel protein 1 antibody, anti-Outer mitochondrial membrane protein porin 1 antibody, anti-Plasmalemmal porin antibody, anti-MGC111064 antibody, anti-PORIN antibody, anti-PORIN-31-HL antibody, anti-VDAC-1 antibody, anti-hVDAC1 antibody, anti- Outer mitochondrial membrane protein porin 1 antibody, anti-Porin 31HL antibody, anti-Porin 31HM antibody |
| ProteinTarget: | VDAC1 / Porin antibody - Mitochondrial Loading Control |
| PackageSize: | 0.1 mg |
| Homology: | 100% homology with mouse, avian, mammalian, aquatic, and reptilian proteins. |
| Marker: | Mitochondrial Marker |
| GeneralRef: | 1. Liu, T., et al. Rapid induction of mitochondrial events and caspase-independent apoptosis in Survivin-targeted melanoma cells. Oncogene 23: 39-48 (2004). 2. Suarez, J., et al. In vivo adenoviral transfer of sorcin reverses cardiac contractile abnormalities of diabetic cardiomyopathy. Am. J. Physiol. Heart Circ. Physiol. 286: H68-75 (2004). 3. Leary, S.C., et al. Human SCO1 and SCO2 have independent, cooperative functions in copper delivery to cytochrome c oxidase. Hum. Mol. Genet. 13: 1839-48 (2004). 4. Jonas, E.A., et al. Proapoptotic N-truncated BCL-xL protein activates endogenous mitochondrial channels in living synaptic terminals. Proc. Natl. Acad. Sci. USA 101: 13590-5 (2004). 5. Liu, J., et al. Phospholipid scramblase 3 is the mitochondrial target of protein kinase C delta-induced apoptosis. Cancer Res. 63: 1153-6 (2003). 6. Gustafsson, A.B., et al. TAT protein transduction into isolated perfused hearts: TAT-apoptosis repressor with caspase recruitment domain is cardioprotective. Circulation 106: 735-9 (2002). 7. Le Mellay, V., et al. Negative regulation of mitochondrial VDAC channels by C-Raf kinase. BMC Cell Biol. 3: 14 (2002). |
| NotesMain: | Such broad reactivity makes this antibody useful as an excellent loading control with whole cell lysates and total mitochondrial preparations. |
|