| CatalogCode: | NB100-694 |
| ProductName: | JAK 3 Antibody |
| Product Description: | Mouse Monoclonal anti-JAK 3 (A1-14-16) |
| Clone: | B26/12/1995 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein, corresponding to amino acids 1076-1100 (C-terminus) of Mouse JAK3. |
| Specificity: | This antibody is specific for JAK 3 and does not cross react with JAK 1, JAK 2 or Tyk 2. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Uses: | Immunohistochemistry (formalin fixed paraffin embedded): use at a dilution of 1:25 - 1:50 in an ABC method. Final dilutions may vary depending upon the staining conditions and the sample.Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Not tested in other applications. |
| Localization: | Cell membrane, cytoplasmic |
| Control: | Colon carcinoma. |
| Background: | JAK (Janus or Just Another Kinase 1) is one of a number of related tyrosine kinases involved in cytoplasmic signal transduction. In response to a variety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues as well as perhaps one or more serine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activators of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK3 has been found predominantly in hematopoietic cells. Recently, it has also been located in normal and transformed human cell types. JAK2 is required for the interferon (IFN) gamma-receptor complex initiation; however, some studies have suggested that the role of JAK2 might be performed by protein tyrosine kinase (Tyk2) and JAK3 if they were positioned correctly within the IFN gamma complex. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2a kappa kappa |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| ListPrice: | 285 |
| AppSummary: | IHC-P |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-JAKL antibody, anti-Janus Kinase 3 antibody, anti-Leukocyte janus kinase antibody, anti-LJAK antibody, anti-JAK3 antibody, anti-JAK-3 antibody |
| ProteinTarget: | JAK 3 antibody [A1-14-16] |
| PackageSize: | 0.5 ml |
| GeneralRef: | 1. Gurniak CB & Berg LJ Murine JAK3 is preferentially expressed in hematopoietic tissues and lymphocyte precursor cells. Blood 87:3151-60 (1996). |