| CatalogCode: | NB100-68142 |
| ProductName: | ITCH Antibody |
| Product Description: | Goat Polyclonal anti-ITCH / AIF4 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide, C-EIKSHDLKPNGGN, representing the internal region of the sequence according to NP_113671.3 |
| Epitope: | EIKSHDLKPNGGN |
| CrossReactivity: | Human. Other species not tested. Expected based upon sequence homology: Mouse, Rat, Dog |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration: 0.03-0.1ug/ml. |
| Control: | Human Brain (Frontal Cortex) |
| Background: | Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ITCH antibody, anti-itchy homolog E3 ubiquitin protein ligase (mouse) antibody, anti-AIF4 antibody, anti-AIP4 antibody, anti-NAPP1 antibody, anti-dJ468O1.1 antibody, anti-NFE2-associated polypeptide 1 antibody, anti-atrophin-1 interacting protein 4 antibody, anti-dJ468O1.1 (atrophin 1 interacting protein 4, AIP4) antibody, anti-itchy homolog E3 ubiquitin protein ligase antibody, anti-ubiqui antibody |
| ProteinTarget: | ITCH |
| PackageSize: | 0.1 mg |
| Homology: | Human, Mouse, Rat, Dog |
| GeneralRef: | Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination.Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19. PMID: 17115028 |
| Gene_Id: | 83737; 16396 (mouse); 311567 (rat) |
| Gene_Symbol: | ITCH |
| Ref_Sqnce: | NP_113671.3 |
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