| CatalogCode: | NB100-667 |
| ProductName: | APC Antibody |
| Product Description: | Mouse Monoclonal anti-APC (ALi 12-28) |
| Clone: | ALi 12-28 |
| Clonality: | Monoclonal |
| Immunogen: | Amino acids 1-433 N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) fused to maltose binding protein. |
| Epitope: | ALI-12-28 was epitope mapped by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test and was found to bind to APC in the region between nucleotides 135 and 422 (exons 2-3) (Efstathiou et al.). |
| Specificity: | This antibody recognises the N-terminal region of the APC protein. |
| CrossReactivity: | Cross-reacts with Human. Does not cross-react with Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | This antibody has been used routinely for Western blotting on colon cell line HCT116, SW480 and SW837 extracts. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. |
| ProductRef: | 1. Abutaily AS et al. Cadherins, catenins and APC in pleural malignant mesothelioma. J Pathol 201:355-62 (2003). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu(-) |
| ALTnames: | anti-Adenomatous Polyposis Coli antibody; anti-APC antibody; anti-DP2 antibody; anti-DP2.5 antibody; anti-DP3 antibody; anti-FAP antibody; anti-FPC antibody; anti-GS antibody |
| ProteinTarget: | APC [ALi 12-28] Antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Fodde R <i>et al.</i> Mutations in the APC tumour suppressor gene cause chromosomal instability. <i>Nat Cell Biol</i> <b>3</b>:433-8 (2001). <PUBMED:11283620> 2. Efstathiou JA <i>et al.</i> Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells. <i>Proc Natl Acad Sci U S A</i> <b>95</b>:3122-7 (1998). <PUBMED:9501226> |