| CatalogCode: | NB100-66618 |
| ProductName: | SYK Antibody |
| Product Description: | Mouse Monoclonal anti-SYK (SYK-01) |
| Clone: | SYK-01 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant fragment corresponding to amino acids 5 - 360 of human syk. |
| Specificity: | NB100-66618 recognizes protein tyrosine kinase p72syk (syk), a 72kDa non-receptor tyrosine kinase, critical for immunoreceptor signaling in haematopoietic cells, but also expressed by nonhaematopoietic cells, including fibroblasts, endothelial cells and hepatocytes.Syk plays an important role in mast cell response, initiated by the aggregation of the IgE high affinity receptor Fc epsilon R1. Phosphorylation of ITAMs within Fc receptor R1 by another tyrosine kinase lyn, results in the recruitment of syk, which is then responsible for the further phosphorylation of numerous target proteins within many different signal transduction pathways.Further studies have shown that syk also plays a major role in B cell receptor-mediated apoptosis, proliferation and haematopoiesis. Syk is also reported to associate with protein kinase C alpha during platelet activation. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | This antibody is useful for Western Blot, immunoprecipitation |
| Localization: | Cytoplasmic: T cell receptor complex |
| Background: | Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline pH7.4 |
| ListPrice: | 285 |
| AppSummary: | WB, IP |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Spleen tyrosine kinase antibody, anti-Tyrosine protein kinase SYK antibody |
| ProteinTarget: | SYK |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Amoui, M. et al. (1997) Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur. J. Immunol. 27(1): 321-328. 2. Tolar, P. et al. (1997) Protein tyrosine kinase Syk is involved in Thy-1 signaling in rat basophilic leukemia cells. Eur. J. Immunol. 27(12): 3389-3397. 3. Manetz, T.S. et al. (2001) Vav1 regulates phospholipase C gamma activation and calcium responses in mast cells. Mol. Cell. Biol. 21(11): 3763-3774. 4. Swann, P.G.et al. (1999) Requirement for a negative charge at Threonine 60 of the FcR gamma for complete activation of Syk. J. Biol. Chem. 274(33): 23068-23077. 5. Arudchandran, R.et al. (2000) The src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH2-terminal kinase 1. J. Exp. Med. 191(1): 47-59. |
| NotesMain: | Western Blotting: Detects a band of approximately 72kDa in Ramos & RBL cell lysates. |
order or
more information: | company product webpage |
| request information: |
|