| CatalogCode: | NB100-66615 |
| ProductName: | SLP-76 Antibody |
| Product Description: | Mouse Monoclonal anti-SLP-76 (SLP-76/03) |
| Clone: | SLP-76/03 |
| Clonality: | Monoclonal |
| Immunogen: | A fusion protein corresponding to amino acids 216-434 of human SLP-76 |
| Specificity: | NB100-66615 recognises the SH2 domain-containing leucocyte protein (SLP-76), a 76kDa tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage.SLP-76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through GRB2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP-76 include phospholipase C (PLC)-gamma, Vav, Nck and fyn binding protein. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | This antibody is useful for Western Blot, immunoprecipitation |
| Localization: | Cytoplasmic |
| Background: | SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline pH7.4 |
| ListPrice: | 285 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-76 kDa tyrosine phosphoprotein antibody, anti-LCP 2 antibody, anti-Lymphocyte Cytosolic Protein 2 antibody, anti-SH2 Domain Containing Leukocyte Protein 76 KD antibody, anti-SH2 domain containing leukocyte protein of 76kD antibody, anti-SH2 domain containing leukocyte protein of 76kDa antibody, anti-SLP 76 antibody, anti-SLP 76 tyrosine phosphoprotein antibody, anti-SLP76 tyrosine phosphoprotein antibody |
| ProteinTarget: | SLP-76 |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Bonilla, F. A. et al. (2000) Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fc gamma receptors I and II/III. PNAS 97: 1725-1730. 2. Pivniouk, V.I. et al. (1999) SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells. J. Clin. Invest. 103: 1737-1743. 3. Delaguillaumie, A. et al. (2002) Rho GTPases link cytoskeletal rearrangements and activation processes induced via the tetraspanin CD82 in T lymphocytes. J. Cell Science 115: 433-443. 4. Di Bartolo, V et al. (2007) A novel pathway down-modulating T cell activation involves HPK-1 dependent recruitment of 14-3-3 proteins on SLP-76. J. Exp. Med. 204: 681-691. |
| NotesMain: | Western Blotting: Detects a band of approximately 76kDa in T lymphocyte cell lysates. |
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