| CatalogCode: | NB100-66612 |
| ProductName: | p21/WAF1 Antibody |
| Product Description: | Mouse Monoclonal anti-p21/WAF1 (WA-1) |
| Clone: | WA-1 |
| Clonality: | Monoclonal |
| Immunogen: | Human p21 protein |
| Specificity: | NB100-66612 recognises the human cyclin-dependent kinase inhibitor p21/WAF1, which is an important cell cycle regulatory protein, also known as CIP1.The p21 protein inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli.NB100-66612 detects a band of approximately 21kD in MCF-7 cell lysates following UV radiation. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.1 mg of ammonium sulphate precipitation Purified IgG |
| Uses: | This antibody is useful for Western Blot, immunoprecipitation |
| Background: | Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and overexpression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline pH7.4 |
| ListPrice: | 285 |
| AppSummary: | WB, IP |
| SpeciesSummary: | Hu |
| ALTnames: | anti-p21 antibody, anti-Activating Fragment 1 antibody, anti-CAP20 antibody, anti-Cation chloride cotransporter-interacting protein 1 antibody, anti-CDK Interacting Protein 1 antibody, anti-CDKN 1 antibody, anti-CDKN1 antibody, anti-CDKN1A antibody, anti-CIP1 antibody, anti-Cyclin Dependent Kinase Inhibitor 1A antibody, anti-DNA Synthesis Inhibitor antibody, anti-MDA 6 antibody, anti-MDA6 antibody, anti-Melanoma Differentiation Associated Protein 6 antibody, anti-p21 antibody, anti-p21CIP1 antibody, anti-PIC1 antibody, anti-SDI1 antibody, anti-SLC12A9 antibody, anti-WAF1 antibody, anti-Wildtype p53 Activating Fragment 1 antibody |
| ProteinTarget: | p21/WAF1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Horky, M. et al. (1997) Induction of cell-cycle inhibitor p21 in rat ventricular monocytes during early postnatal transition from hyperplasia to hypertrophy. Physiol. Res. 46: 233-235. 2. Kotala, V. et al. (2001) Potent induction of wild-type p53-dependent transcription in tumour cells by a synthetic inhibitor of cyclin-dependent kinase. Cell. Mol. Life Sci. 58: 1333-1339. 3. Kolar, Z. et al. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer. Mol. Pathol. 53: 15-18. 4. Khanna, S.J. et al. (2001) V-Abl protein-tyrosine kinase up-regulates p-21 WAF-1 in cell cycle arrested and proliferating myeloid cells. J. Biol. Chem. 276: 11143-11150. 5. Scott, M.T. et al. (2002) PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein. EMBO J. 21: 6771-6780. |
| NotesMain: | Immunohistology: *This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. |
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