| CatalogCode: | | NB100-66582 |
| ProductName: | | SMAC/DIABLO Antibody |
| Product Description: | | Rabbit Polyclonal anti-SMAC/DIABLO |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide corresponding to amino acids 222-237 of mouse Smac/DIABLO |
| Specificity: | | NB100-66582 recognises the C-terminal region of murine Smac/DIABLO. Inhibitors of apoptosis proteins (IAP's) block cell death by caspase inhibition. X-linked inhibitor of apoptosis (XIAP) is a member of the IAP family. Smac/DIABLO is expressed in a variety of tissues and has been identified as a negative regulator of XIAP. |
| CrossReactivity: | | Mouse. Not yet tested in other species. |
| Packaging: | | 0.1 mg of affinity chromatography Purified IgG |
| Uses: | | This antibody is useful for Western Blot, Immunohistochemistry-Paraffin |
| Localization: | | Mitochondrial but released into the cytosol when cells undergo apoptosis. |
| Background: | | Inhibitors of apoptosis proteins (IAP's) block cell death by caspase inhibition. X linked inhibitor of apoptosis (XIAP) is a member of the IAP family. SMAC/DIABLO (second mitochondria derived activator of caspase/direct IAP binding protein with low PI) is expressed in a variety of tissues, and has been identified as a negative regulator of XIAP. Smac/Diablo is a mitochondrial protein that enters the cytosol during apoptosis and functions as a regulatory component to moderate the caspase inhibition of IAPs. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Phosphate buffered saline |
| ListPrice: | | 285 |
| AppSummary: | | WB, IHC-P |
| SpeciesSummary: | | Mu |
| ALTnames: | | anti-0610041G12Rik antibody, anti-DBOH antibody, anti-Diablo homolog (Drosophila) antibody, anti-Diablo homolog antibody, anti-Diablo homolog mitochondrial antibody, anti-DIABLO S antibody, anti-Direct IAP binding protein with low pI antibody, anti-FLJ10537 antibody, anti-FLJ25049 antibody, anti-Mitochondrial Smac protein antibody, anti-Second mitochondria derived activator of caspase antibody, anti-SMAC 3 antibody, anti-Smac protein antibody, anti-SMAC3 antibody |
| ProteinTarget: | | SMAC/DIABLO (C-TERMINAL) |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | 1. Verhagen, A. M. et al (2001) Inhibitor of apoptosis proteins and their relatives; IAP's and other BIRPS's. Genome Biol. 2(7): Reviews 3009. 2. Wu, G. et al (2000) Structural basis of IAP recognition by Smac/DIABLO. Nature. 408: 1008-12 |
| NotesMain: | | Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. |
| more information: | | company product webpage |