| CatalogCode: | | NB100-66560 |
| ProductName: | | AIF Antibody |
| Product Description: | | Rabbit Polyclonal anti-AIF |
| Clonality: | | Polyclonal |
| Immunogen: | | Peptide corresponding to amino acids 517 - 531 of human AIF. |
| Specificity: | | AHP578 recognises apoptosis inducing factor (AIF). The antibody reacts with an internal region of AIF. AIF is widely expressed in human tissues and localises in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. |
| CrossReactivity: | | Reacts with: Rat, Mouse |
| Packaging: | | 0.1 mg of ion exchange chromatography Purified IgG |
| Uses: | | This antibody is useful for Western Blot, Immunohistochemistry-Paraffin |
| Localization: | | Mitochondrial intermembrane space. Translocated to the nucleus upon induction of apoptosis. |
| Background: | | Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase 9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Phosphate buffered saline |
| ListPrice: | | 285 |
| AppSummary: | | WB, IHC-P |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Apoptosis inducing factor antibody, anti-Harlequin antibody, anti-Hq antibody, anti-mAIF antibody, anti-MGC111425 antibody, anti-MGC5706 antibody, anti-PDCD 8 antibody, anti-PDCD8 antibody, anti-Programmed cell death 8 antibody, anti-Programmed cell death 8 isoform 1 antibody, anti-Programmed cell death 8 isoform 2 antibody, anti-Programmed cell death 8 isoform 3 antibody, anti-Programmed cell death protein 8 mitochondrial antibody, anti-Programmed cell death protein 8 mitochondrial precursor antibody, anti-Striatal apoptosis inducing factor antibody |
| ProteinTarget: | | AIF (aa517-531) |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | 1. Susin. S.A. et al. (1999) Molecular characterization of mitochondrial apoptosis - inducing factor. Nature. 397: 441-6. |
| NotesMain: | | Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. |
| more information: | | company product webpage |
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