| CatalogCode: | NB100-663 |
| ProductName: | eIF-2A Antibody |
| Product Description: | Mouse Monoclonal anti-eIF-2A (EIF2a) |
| Clone: | EIF2a |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | This antibody recognizes the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2A). |
| CrossReactivity: | Human, Mouse, and Rat. Other species have not been tested. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | This antibody is useful for Western blot, in which a band of approximately 36 kDa is seen. |
| Localization: | Cytoplasmic |
| Control: | Human - Jurkat, CEM and HeLa cells. Mouse - 3T3L1 cells Rat - PC-12 cells. |
| Background: | eIF-2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF-2 alpha is phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2 alpha blocks the GDP-GTP exchange activity of eIF-2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF-2 alpha is an important regulatory process in protein synthesis. |
| ProductRef: | 1. Ramos-Castaneda, J., et al. Deficiency of ATP2C1, a Golgi ion pump, induces secretory pathway defects in endoplasmic reticulum (ER)-associated degradation and sensitivity to ER stress. J. Biol. Chem. 280: 9467-73 (2005). [PubMed: 15623514] 2. Trottier, M.D., et al. VSV replication in neurons is inhibited by type I IFN at multiple stages of infection. Virology 333: 215-25 (2005). [PubMed: 15721356] |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS, [pH 7.4] |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-Eukaryotic Translation Initiation Factor 2 alpha antibody, anti-CDA 02 antibody, anti-CDA02 antibody, anti-EIF-2alpha antibody, anti-eIF2S1 antibody |
| ProteinTarget: | eIF2alpha |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Patel C.V., et al. PACT, a stress-modulated cellular activator of interferon-induced double-stranded RNA-activated protein kinase, PKR. J. Biol. Chem. 275: 37993-8 (2000). [PUBMED:10988289] 2. Datta B., et al. Increased phosphorylation of eukaryotic initiation factor 2alpha at the G2/M boundary in human osteosarcoma cells correlates with deglycosylation of p67 and a decreased rate of protein synthesis. Exp. Cell Res. 250: 223-30 (1999). [PUBMED:10388536] 3. Kimball S.R. Eukaryotic initiation factor eIF2. Int. J. Biochem. Cell Biol. 31: 25-9 (1999). [PUBMED:10216940] 4. Ernst H., et al. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. J. Biol. Chem. 262: 1206-12 (1987). [PUBMED:2948954] 5. Lu, J., et al. The interferon-induced double-stranded RNA-activated protein kinase PKR will phosphorylate serine, threonine, or tyrosine at residue 51 in eukaryotic initiation factor 2alpha. J. Biol. Chem. 274(45): 32198-32203, (1999). 6. Kimball, S.R., et al. Purification and characterization of eukaryotic translational initiation factor eIF-2B from liver. Biochem. Biophys. Acta. 1201(3): 473-481, (1994). 7. Pavitt, G.D., et al. eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange. Genes Dev. 12(4): 514-526, (1999). 8. Zhang, P.C., et al. The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system. postnatal growth, and the function and viability of the pancreas. Mol. Cell. Biol. 22(11): 3864-3874, (2002). |
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