| CatalogCode: | | NB100-66073 |
| ProductName: | | CASPASE-8 Antibody |
| Product Description: | | Rabbit Polyclonal anti-CASPASE-8 |
| Clonality: | | Polyclonal |
| Immunogen: | | A 16 amino acid peptide located near the carboxy-terminus of human caspase-8 isoform A. |
| Specificity: | | NB100-66073 recognises an epitope within the c-terminal region (CT) of caspase-8, otherwise known as Fas-linked ICE-like protease (FLICE), which shares homology with both Fas-associated via death domain (FADD) and the ICE/Ced-3 family of cysteine proteases.Caspase-8 is classified as an initiator caspase and acts as the most upstream trigger of Fas-Receptor (CD95) and tumour necrosis factor receptor 1 (TNFR1) mediated apoptosis. Upon activation, the cell death receptor Fas, links with the adaptor molecule FADD via corresponding death domains (DD). FADD in turn then binds via death effector domains (DEDs) to procaspase-8, which then undergoes oligomerization and autocatalytic activation.Active Caspase-8 is capable of cleaving all other known caspases, and also cleaves the Bcl-2 family member BH3-interacting domain death agonist (BID), along with the Bcl-2 associated protein p28Bap31(2). |
| CrossReactivity: | | Reacts with: Mouse, Rat |
| Packaging: | | 0.1 mg of affinity chromatography Purified IgG |
| Uses: | | This antibody is useful for Western Blot |
| Localization: | | Cytoplasmic |
| Background: | | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Phosphate buffered saline |
| ListPrice: | | 285 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Apoptosis related cysteine peptidase antibody, anti-Apoptotic cysteine protease antibody, anti-Apoptotic protease Mch 5 antibody, anti-CAP 4 antibody, anti-CAP4 antibody, anti-CASP 8 antibody, anti-CASP8 antibody, anti-Caspase8 antibody, anti-CED 3 antibody, anti-FADD homologous ICE/CED 3 like protease antibody, anti-FADD Homologous ICE/CED3 Like Protease antibody, anti-FADD Like ICE antibody, anti-FLICE antibody, anti-ICE like apoptotic protease 5 antibody, anti-MACH antibody, anti-MCH 5 antibody, anti-MCH5 antibody, anti-MGC78473 antibody, anti-MORT1 associated CED 3 homolog antibody, anti-MORT1 associated CED3 homolog antibody |
| ProteinTarget: | | CASPASE-8 (C-TERMINAL) |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | 1. Muzio, M. et al. (1996) FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signalling complex. Cell 85: 817 - 827. 2. Wolf, B. B. and Green, D. R. (1999) Suicidal tendancies: Apoptotic cell death by caspase family proteinases. J. Biol. Chem. 274: 20049 - 20052. 3. Cohen, G. M. (1997) Caspase: the executioners of apoptosis. Biochem. J. 326: 1 - 16. |
| NotesMain: | | Western Blotting: Detects a cleaved subunit band of approximately 22.5kDa in Jurkat cell lysates (predicted precursor MWt 57.7kDa). |
| more information: | | company product webpage |
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