| CatalogCode: | NB100-66067 |
| ProductName: | CAD Antibody |
| Product Description: | Rabbit Polyclonal anti-CAD |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide corresponding to amino acids 205-222 of mouse CAD |
| Specificity: | CAD (Caspase Activated Deoxyribonuclease) is the murine homologue of human DFF-40.CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA.NB100-66067 recognises the 40kD CAD in Western blots. |
| CrossReactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg of affinity chromatography Purified IgG |
| Uses: | This antibody is useful for Western Blot |
| Localization: | Cytoplasmic |
| Background: | Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Phosphate buffered saline |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Mu |
| ALTnames: | anti-Aspartate carbamoyltransferase antibody, anti-Aspartate transcarbamylase antibody, anti-CAD protein antibody, anti-CAD trifunctional protein antibody, anti-Carbamoyl phosphate synthetase 2 antibody, anti-CPSase ATCase DHOase antibody, anti-Dihydroorotase antibody, anti-Glutamine dependent carbamoyl phosphate synthase antibody, anti-Multifunctional protein CAD antibody |
| ProteinTarget: | CAD (aa205-222) |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Enari, M. et al. (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391: 43-50. 2. Sakahira, H. et al. (1998) Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 391: 96-99. 3. Liu, X. et al. (1997) DFF, a heterodimeric protein that functions downsram of Caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89: 175-184. |